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KMID : 0900919910150010033
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Korean journal of Animal Reproduction
1991 Volume.15 No. 1 p.33 ~ p.39
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Morphological Changes of Bovine Epididymal Spermatozoa Incubated In Chemical Defined Medium
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Ko Dae-Hwan
Lee Kyung-Kwang
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Abstract
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This experiment was carried out to investigate ultrastructural changes that occurred in bovine epididymal spermatozoa during incubation in a BO medium supplemented with 5 mM caffeine. No structural changes were observed under electron microscope in the majority of non-incubated sperm(79.8%) keeping the membrane intact. Structural changes were however observed when spermatozoa were cultured in a BO medium supplemented with 5 mM caffeine, which were classified into 4 types, intact(29.4%), vesiculated(45.6%), acrosome lost(17.8%) and degenerated(7.2%) spermatozoa, respectively. These results indicated that : 1) vesiculation of spermatozoa membrane is normal acrosome reaction and acrosome lost of spermatozoa is dead one ; 2) caffeine can induce acrosome reaction of bovine epididymal spermatozoa.
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KEYWORD
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